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Structured Review

Syndax Inc entinostat
Entinostat, supplied by Syndax Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/entinostat/entinostat/us12655159-432-22-24
Average 86 stars, based on 1 article reviews
entinostat - by Bioz Stars, 2026-10
86/100 stars

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Western Blot:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Transplantation Assay:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

RNA Sequencing:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Expressing:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Flow Cytometry:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Co-Culture Assay:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Single Cell:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Isolation:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Derivative Assay:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Fluorescence:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Magnetic Beads:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Activation Assay:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

ChIP-sequencing:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).

Live Cell Imaging:

Article Title: Heterobifunctional compounds and their use in treating disease
Article Snippet: Other HDAC inhibitors being studied which may be used in the present invention include entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).

Article Title: Differential cell signaling testing for cell-cell communication inference from single-cell data by dominoSignal.
Article Snippet: We would like to thank Dr. Peter Ordentlich of Syndax for providing the Entinostat utilized in this study.

Article Title: HDAC Inhibition Sensitizes Pancreatic Tumors to DNA Damage by Global Redistribution of the Transcriptional Machinery
Article Snippet: R.M.E. and M.D. are co-founders of Syndax, which holds the rights to entinostat.

Article Title: SMARCA degraders and uses thereof
Article Snippet: In some embodiments, a CDK 4/6 inhibitor is selected from palbociclib (Ibrance, Pfizer); ribociclib (Kisqali, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).

Article Title: A class act: HDAC1- Malat1 regulates MDSC apoptosis and cell cycling to decrease suppression of T cells
Article Snippet: Chemical inhibitors used in this study: Entinostat (Syndax Pharmaceuticals), Panobinostat (MedChemExpress, cat. #HY-10224), Belinostat (MedChemExpress, cat. #HY-10225), Vorinostat (MedChemExpress, cat. #HY-10221), Trichostatin A (MedChemExpress, cat. #HY-15144), Domatinostat (MedChemExpress, cat. #HY-16012A), Chlopynostat (MedChemExpress, cat. #HY-161464), Pyroxamide (MedChemExpress, cat. #HY-13216), Santacruzamate A (MedChemExpress, cat. #HY-N0931), RGFP966 (MedChemExpress, cat. #HY-13909), TMP269 (MedChemExpress, cat. #HY-18360), SIS17 (MedChemExpress, cat. #HY-128918), Stattic (MedChemExpress, cat. #HY-13818), Abemaciclib (MedChemExpress, cat. #HY-16297A), Palbociclib (MedChemExpress, cat. #50767).



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(A) Dose-response curves for ATRT cell lines treated with corin for 72 h (n=3). (B) Relative cell viability of ATRT cell lines treated with corin versus the HDAC inhibitor, entinostat/ MS-275 (n=3). (C) Table showing comparative IC 50 s of corin and entinostat in ATRT cell lines sorted by molecular subtype. IC 50 was calculated based on the total viable cell count relative to DMSO in ATRT cell lines. (D) BrdU immunostaining and quantification of ATRT cells treated with DMSO or corin (n=5). Scale 50 μm. (E) Cleaved caspase 3 (CC3) immunostaining for apoptosis and quantification of ATRT cells treated with DMSO versus corin (n=5). Scale 50 μm. (F) MUSE Annexin V/ 7-AAD assay of ATRT cells treated with DMSO or corin (n=3). (G) Quantification of Annexin V apoptosis assay for corin treatment of ATRT cells in (F). Data is presented as fold change of the total number of ATRT cells undergoing apoptosis following corin treatment relative to DMSO. (H) Western blot of cPARP expression in ATRT cells following 72 h treatment with DMSO versus corin (n=3). The band density normalized to beta actin and relative to DMSO is shown below each band. Statistical Analysis: unpaired, two-tailed student’s t test. All data represents mean ± SD. ** p ≤ 0.01; *** p ≤ 0.001.
Hdac Inhibitor Entinostat Ms 275, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress entinostat
HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with <t>entinostat.</t> (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .
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HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with <t>entinostat.</t> (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .
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HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with <t>entinostat.</t> (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .
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HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with <t>entinostat.</t> (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .
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(A) Dose-response curves for ATRT cell lines treated with corin for 72 h (n=3). (B) Relative cell viability of ATRT cell lines treated with corin versus the HDAC inhibitor, entinostat/ MS-275 (n=3). (C) Table showing comparative IC 50 s of corin and entinostat in ATRT cell lines sorted by molecular subtype. IC 50 was calculated based on the total viable cell count relative to DMSO in ATRT cell lines. (D) BrdU immunostaining and quantification of ATRT cells treated with DMSO or corin (n=5). Scale 50 μm. (E) Cleaved caspase 3 (CC3) immunostaining for apoptosis and quantification of ATRT cells treated with DMSO versus corin (n=5). Scale 50 μm. (F) MUSE Annexin V/ 7-AAD assay of ATRT cells treated with DMSO or corin (n=3). (G) Quantification of Annexin V apoptosis assay for corin treatment of ATRT cells in (F). Data is presented as fold change of the total number of ATRT cells undergoing apoptosis following corin treatment relative to DMSO. (H) Western blot of cPARP expression in ATRT cells following 72 h treatment with DMSO versus corin (n=3). The band density normalized to beta actin and relative to DMSO is shown below each band. Statistical Analysis: unpaired, two-tailed student’s t test. All data represents mean ± SD. ** p ≤ 0.01; *** p ≤ 0.001.

Journal: Neuro-oncology

Article Title: The CoREST complex inhibitor, corin, decreases tumor growth, increases cellular differentiation and extends lifespan in ATRT xenograft models

doi: 10.1093/neuonc/noag160

Figure Lengend Snippet: (A) Dose-response curves for ATRT cell lines treated with corin for 72 h (n=3). (B) Relative cell viability of ATRT cell lines treated with corin versus the HDAC inhibitor, entinostat/ MS-275 (n=3). (C) Table showing comparative IC 50 s of corin and entinostat in ATRT cell lines sorted by molecular subtype. IC 50 was calculated based on the total viable cell count relative to DMSO in ATRT cell lines. (D) BrdU immunostaining and quantification of ATRT cells treated with DMSO or corin (n=5). Scale 50 μm. (E) Cleaved caspase 3 (CC3) immunostaining for apoptosis and quantification of ATRT cells treated with DMSO versus corin (n=5). Scale 50 μm. (F) MUSE Annexin V/ 7-AAD assay of ATRT cells treated with DMSO or corin (n=3). (G) Quantification of Annexin V apoptosis assay for corin treatment of ATRT cells in (F). Data is presented as fold change of the total number of ATRT cells undergoing apoptosis following corin treatment relative to DMSO. (H) Western blot of cPARP expression in ATRT cells following 72 h treatment with DMSO versus corin (n=3). The band density normalized to beta actin and relative to DMSO is shown below each band. Statistical Analysis: unpaired, two-tailed student’s t test. All data represents mean ± SD. ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: The HDAC inhibitor entinostat/MS-275 (Cat# HY-12163) and corin (Cat# HY-111048) were purchased from MedChemExpress (NJ, USA).

Techniques: In Vitro, Cell Characterization, Immunostaining, Apoptosis Assay, Western Blot, Expressing, Analysis, Two Tailed Test

HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with entinostat. (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .

Journal: Advanced Science

Article Title: SPSB1 Promotes Subcutaneous Adipose Hyperplasia in Facial Port‐Wine Stains by Controlling HDAC1 Degradation and Stability Through Two Distinct Proteolytic Pathways

doi: 10.1002/advs.76699

Figure Lengend Snippet: HDAC1 regulated adipogenesis by transcriptional reprogramming. (A): Volcano plot of significantly upregulated (red) and downregulated (blue) genes identified by comparing si‐HDAC1 or si‐Control treated PWS‐ASPCs. (B): Heatmaps showed the RNA‐seq results of differentially expressed genes related to regulation of lipid metabolic process in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (C): Heatmaps showed the RNA‐seq results of differentially expressed genes related to lipid transport in si‐HDAC1 or si‐Control treated PWS‐ASPCs. (D): Gene set enrichment analysis plot of genes related to adipogenesis that were enriched in PWS‐ASPCs with HDAC1 knockdown. (E): Dot plot showed the significant Kyoto Encyclopedia of Genes and Genomes pathways among the differentially expressed genes. (F): WB and quantitative analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs with or without HDAC1 knockdown. (G): WB analysis for the expression of H3K27ac and H3K9ac in PWS‐ASPCs treated with entinostat. (H): Heatmaps for differentially enriched ATAC‐seq peaks in PWS‐ASPCs with or without HDAC1 knockdown. (I): Pie chart showed the genomic distribution of accessible regions. (J): Motif enrichment analysis of differentially accessible chromatin regions in HDAC1‐knockdown PWS‐ASPCs. This analysis reported absolute motif frequencies within differential peaks and did not provide fold enrichment relative to controls due to methodological limitations of standard motif discovery algorithms. (K): IGV maps showed the increased ATAC‐seq peaks of representative adipogenic genes. (L): Heatmaps showed the ATAC‐seq analysis of upregulated genes related to fat cell differentiation. (M): Quadrantal diagram depicting the overlap of significantly differentially expressed genes from bulk RNA‐seq and genes with significantly variational peaks from ATAC‐seq data. Both upregulated (orange); both downregulated (blue). Data were analyzed by unpaired two‐sided Student's t tests (F, G) and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .

Article Snippet: When required, Induction solution A and B were supplemented with Entinostat (MedChemExpress, HY‐111770).

Techniques: Control, RNA Sequencing, Knockdown, Expressing, Cell Differentiation

SPSB1‐HDAC1 positive feedback loop regulated adipogenesis. (A): Heatmaps and the peaks intensity quantitative analysis showed the chromatin accessibility of SPSB1 in ATAC‐seq. (B): IGV maps showed the increased ATAC‐seq peaks of SPSB1 promoter. (C): H3K27ac and H3K9ac ChIP‐qPCR validation for SPSB1 promoter regions in PWS‐ASPCs with different interventions. (D): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with or without HDAC1 knockdown. (E): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with or without HDAC1 overexpression. (F): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with different dose of entinostat administration. (G, H): H&E staining of Matrigel implants collected on day 28 and quantification of adipocytes size. Scale bar: up: 400 µm; down: 100 µm. (I, J): Oil Red O staining of Matrigel implants and quantification of Oil red O staining area. Scale bar: up: 400 µm; down: 100 µm. (K, L): The protein level of PPAR γ detected by IHC in the implants of each group. Scale bar: Scale bar: up: 400 µm; down: 100 µm. (M): Statistical analysis of adipocyte size in (G). (N): Statistical analysis of Oil red O staining area in (I). (O): Statistical analysis of PPAR γ intensity in (K). (P): Statistical analysis of adipocyte size in (H). (Q): Statistical analysis of Oil red O staining area in (J). (R): Statistical analysis of PPAR γ intensity in (L). Data were analyzed by unpaired two‐sided Student's t tests (D, E) or one‐way ANOVA (F, M, N, O, P, Q, R), and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .

Journal: Advanced Science

Article Title: SPSB1 Promotes Subcutaneous Adipose Hyperplasia in Facial Port‐Wine Stains by Controlling HDAC1 Degradation and Stability Through Two Distinct Proteolytic Pathways

doi: 10.1002/advs.76699

Figure Lengend Snippet: SPSB1‐HDAC1 positive feedback loop regulated adipogenesis. (A): Heatmaps and the peaks intensity quantitative analysis showed the chromatin accessibility of SPSB1 in ATAC‐seq. (B): IGV maps showed the increased ATAC‐seq peaks of SPSB1 promoter. (C): H3K27ac and H3K9ac ChIP‐qPCR validation for SPSB1 promoter regions in PWS‐ASPCs with different interventions. (D): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with or without HDAC1 knockdown. (E): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with or without HDAC1 overexpression. (F): WB and quantitative analysis showed that the SPSB1 and HDAC1 protein levels in PWS‐ASPCs with different dose of entinostat administration. (G, H): H&E staining of Matrigel implants collected on day 28 and quantification of adipocytes size. Scale bar: up: 400 µm; down: 100 µm. (I, J): Oil Red O staining of Matrigel implants and quantification of Oil red O staining area. Scale bar: up: 400 µm; down: 100 µm. (K, L): The protein level of PPAR γ detected by IHC in the implants of each group. Scale bar: Scale bar: up: 400 µm; down: 100 µm. (M): Statistical analysis of adipocyte size in (G). (N): Statistical analysis of Oil red O staining area in (I). (O): Statistical analysis of PPAR γ intensity in (K). (P): Statistical analysis of adipocyte size in (H). (Q): Statistical analysis of Oil red O staining area in (J). (R): Statistical analysis of PPAR γ intensity in (L). Data were analyzed by unpaired two‐sided Student's t tests (D, E) or one‐way ANOVA (F, M, N, O, P, Q, R), and were presented as mean ± SD with at least three replicate experiments. Original blot can be found in Figure .

Article Snippet: When required, Induction solution A and B were supplemented with Entinostat (MedChemExpress, HY‐111770).

Techniques: ChIP-qPCR, Biomarker Discovery, Knockdown, Over Expression, Staining